Oxazaphosphorine cytostatics: A good way to create better is to study old oxazaphosphorines

Although Cyclophosphamide and other oxazaphosphorine cytostatics are among the oldest and most widely used drugs in cancer chemotherapy, little is known about the cause of the high antitumor activity of this class of alkylating drugs compared to other alkylating cytostatics. In the present article a new scheme for the mechanism of action of oxazaphosphorine cytostatics based on the finding that proapoptotic 2-hydroxypropanal is formed during the enzymatic degradation of aldophosphamide is presented. Based on this scheme, possibilities for improving the antitumor activity are discussed and illustrated with practical examples. Cyclophosphamide (CP) is one of the most effective and most frequently used anticancer agents; but so far little is known about the reason of the relative high anti tumor activity. After hydroxylation of CP by the hepatic P450 enzyme the resulting 4-hydroxycyclophosphamide (OHCP) forms an equilibrium with his tautomeric aldehyde Aldophosphamide (ALDO). OHCP and ALDO are not cytotoxic. In vivo the tautomers OHCP and ALDO are irreversibly detoxified by aldehyde dehydrogenases. It is generally accepted that not detoxified ALDO is spontaneously decomposed to the cytotoxic alkylating phosphoreamide mustard (PAM) and the toxic byproduct acrolein. The common opinion is that PAM unfolds its cell toxicity by inhibition of cell division due to DNA alkylation. Lower activity of aldehyde dehydrogenases in tumor cells and thus higher concentration of OHCP/ALDO is considered to be the reason for the high antitumor activity of CP. This conclusion is based on in vitro experiments in which resistance of tumor cells against OHCP can be reversed by inhibitors of detoxifying aldehyde dehydrogenases [1]. Under physiological conditions however the fate of OHCP / ALDO differs from its fate in vitro. OHCP and ALDO are stable compounds which only release insignificant amounts of PAM (half life of OHCP/ALDO in protein free rat serum ultra filtrate > 20h) On the contrary the half life of OHCP/ ALDO in whole rat serum was determined to be 20 min indicating enzymatic decomposition. The decay catalyzing enzyme was identified to be a phosphodiesterase yielding the decomposition products PAM and 3-hydroxypropanal (HPA) instead of acrolein [2] (Figure 1). Schwartz and Waxman [3] investigated the effect of OHCP on the extrinsic and intrinsic pathways of apoptosis in tumor cells. Contrary to other anticancer drugs like doxorubicin and cisplatin [4,5] which initiate the extrinsic pathway of apoptosis, 4-OHCP initiates the intrinsic, p53 mediated pathway in response to DNA damage. From their findings Schwartz and Waxman postulated a bimodal mechanism of action of OHCP/ALDO on tumor cells. Firstly DNA of cells is damaged by alkylation by PAM and secondly damaged cells are eliminated by apoptosis. HPA known as Reuterin is produced by Lactobacillus reuteri and submitted to the culture medium. It is intensively investigated by Iyer et al. [6] and Wu et al [7]. They showed that reuterin inhibits both the antiapoptotic proteins Bcl-2 and the TNF dependend NF-κB activation. Correspondence to: Georg Voelcker, Institute of Biochemistry II, Goethe University Frankfurt Medical School, Frankfurt, Germany, Tel: +49 (0)69 6301 5652, Fax: +49 (0)69 6301 5577, E-mail: [email protected] / [email protected] key words: oxazaphosphorine cytostatics, 2-hydroxypropanal, perhydrothiazinylphosphamide ester, influence of DNA damage in p53 mediated apoptosis Received: April 07, 2017; Accepted: April 23, 2017; Published: April 26, 2017 Voelcker G (2017) Oxazaphosphorine cytostatics: A good way to create better is to study old oxazaphosphorines Volume 4(2): 2-2 Integr Cancer Sci Therap, 2017 doi: 10.15761/ICST.1000237 model. LD50 for acute toxicity in mice was determined to be 2.3 mmol/ kg for Ifosfamide (IF) compared to 6.4 mmol/kg determined for IAP. IAP is the perhydrothiazinylphosphamide ester of aldo-Ifosfamide (Figure 3). In therapy experiments in P388 tumor bearing mice with equitoxic doses of IF and IAP long time survivors (survival time >100d) were achieved with IAP but not in the experiments with IF. An almost explosive enhancement of the antitumor activity was shown in comparative therapy experiments with equimolar doses of IAP and SUM-IAP in advanced solid growing P388 tumor bearing mice. In SUM-IAP one 2-chloroethyl group of the IF structure of IAP is replaced by a mesylethyl group (Figure 3). While in the experiments with IAP only a marginal delay in tumor growth was measured, the tumor mass in the experiments with SUM-IAP was reduced below detection level [9]. The experiments clearly demonstrate improvement of antitumor activity of oxazaphosphorine cytostatics by bypassing hydroxylation by liver enzymes and optimisation of DNA alkylation.